Thin-Layer Chromatography Kit AP Chemistry

Chromatography is an analytical tool used to separate similar compounds. Thin-Layer Chromatography utilizes a sheet coated with silica gel. The samples are applied to the silica gel sheet and separated by migrating an appropriate solvent up the sheet. This type of separation is called adsorption chromatography because the separation of similar compounds is based on the selective adsorption of the compounds on the silica gel solid phase.

In this lab samples of various plant leaf materials may be extracted or the red and green leaf extracts included may be used. The samples of extracted leaf pigments are applied to a silica gel sheet and separated using a mixture of organic solvents.

Kit Includes:

• Alfalfa Powder - 10 g x 2 Bottles
• Red Extract UN1219 - 25 ml x 2 Bottles
• Chromatography TLC Sheets - 15 pcs
• Chromatographic Solvent UN1230 - 250 ml x 6
• Isopropyl Alcohol, 70% UN1219 - 50 ml x 2

• Chromatography Chambers or (# 6131-6) Beaker 500 ml - 15 pcs
• (# 5510-9) Beral Pipettes 1 ml - 30 pcs

• (# 3776-2) Mortar & Pestle (to grind specimens)
• (# 6135-3) Test Tube 13 x 100 mm (for extractions)

DOT: UN1230, Methanol, 3, PG II, Ltd Qty
UN1219, Isopropanol, 3, PG II, Ltd Qty
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