AP Biology Investigation #7: Cell Division: Mitosis and Meiosis

Students will study the cellular steps involved in DNA replication and cell division in both mitosis and meiosis. This investigation will allow students to examine the stages of mitosis in the preparation of plant root tips. Crossing over in meiosis will also be investigated through the use of Sordaria cultures.

This kit contains enough materials for 32 students in 8 groups.

Meets AP Science Practices 1, 5, 6, and 7, and Big Idea 3.

Kit Includes:

• Disposable scalpels, sterile - 10 pcs
• Petri dishes, sterile - 20 pcs
• Sordaria agar 200 ml
• Sordaria mating agar 200 ml
• Disposable inoculating loops, sterile - 8 pcs
• Forceps - 8 pcs
• Microscope slides - 1 Pack
• Coverslips - 1 Pack
• Plastic pipettes - 16 pcs
• Plastic cups - 8 pcs
• Sand - 500g
• Hydrochloric acid, 6.0M - 30 ml
• Toluidine blue, 1% - 30ml
• Coupon redeemable for:
  • Sordaria, wild type
  • Sordaria, tan mutant

Materials Needed but not Supplied:

• Microscopes
• Distilled water
• Microwave or hot waterbath
• Garlic cloves

Optional:

• Lectin
• pH buffers
• NaCl
• Plant hormones (e.g. indole acetic acid, kinetin)
• Herbicides
• Any other materials students may think might affect the rate of mitosis in garlic root tips

DOT: Small Quantity Exemption 173.4 This Package Conforms to 49 CFR 173.4 for domestic highway or rail transport only

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